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DNA barcoding of cryptogams, including biosystematic studies of selected groups

Department 58 – International Relations
Department 58 – International Relations

Published

Updated 18-9-2017
  • Changes in number and Summary

Project
RegionSouth Moravian Region
Title of the ProgrammeCZ07 - Scholarship Programme
Title of the ProjectDNA barcoding of cryptogams, including biosystematic studies of selected groups
Project Website

https://www.muni.cz/en

Number of the ProjectNF-CZ07-ICP-3-104-2015
Project Promoter

Masaryk University

Name of Norwegian Partner(s)

Norwegian University of Science and Technology

Objective of the Project

This project has established collaboration between Masaryk University (Brno, Czech Republic) and the Norwegian University of Science and Technology (NTNU, Trondheim, Norway). Some additional selected taxonomic experts from other institutions have been associated. The project’s aims have been fulfilled and we have successfully DNA-barcoded approximately 100 taxa of bryophytes and lichens, and established a permanent workstation for DNA-barcoding at Masaryk University. Barcode sequences have been used to study species delimitation in the lichen genera Cladonia, Placynthium, and Solenopsora, and the bryophyte genus Sphagnum. This project was the first attempt to DNA-barcode bryophytes and lichens in the Czech Republic. Additional DNA-regions were produced for performing multi-locus phylogenetic analyses of the selected lichen genera while future collaboration has also been established. Moreover, we have been studying the circumarctic, boreal and temperate populations of Sphagnum warnstorfii using restriction site associated DNA markers (RADseq). The RADseq data was produced through mobilities at the NTNU University Museum, under the supervision of the local experts. The implementation of this NGS approach at Masaryk University was done and knowledge was shared successfully and will continue in the future. This project has increased the level of competence of students and staff of Masaryk University when it comes to DNA barcoding and NGS approaches to biosystematics. It has formed the basis for further collaboration in the future.

Approved grant

1 236 302 CZK

Project DurationStart date: 1.7. 2015, End date: 30.6.2016